Description
Anti-Human IL-4 In Vivo Antibody - Low Endotoxin
Introducing the Anti-Human IL-4 In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets interleukin 4 (IL-4), a critical cytokine in the immune response, making it ideal for research in immunology and related fields.
With a rat IgG1 isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), perfect for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy. Enhance your research with this reliable and versatile antibody. IL-4 is a significant cytokine involved in immune response regulation, playing a crucial role in the differentiation of naive helper T cells (Th0 cells) to Th2 cells. It influences various immune cells, including B cells, T cells, and mast cells, promoting antibody production, particularly immunoglobulin E (IgE), and is pivotal in allergic responses and asthma.
| Product Name: | Anti-Human IL-4 In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0003 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | MP4-25D2 |
| Protein: | IL-4 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | BCGF, BCDF, B Cell Stimulating Factor, BSF-1 |
| Isotype: | Rat IgG1 κ |
| Reactivity: | Human |
| Immunogen: | CHO-expressed, recombinant human IL-4 |
| Applications: | CyTOF®, IHC, N, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | CyTOF®, IHC, N, WB |
| Recommended Usage: | ELISA Each lot of this antibody is quality control tested by ELISA. |
| Reactivity: | Human |
| Host Species: | Rat |
| Specificity: | Clone MP4-25D2 recognizes human IL-4. |
| Immunogen: | CHO-expressed, recombinant human IL-4 |
| Concentration: | ≥ 2.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
IL-4 is a 15-19 kD pleiotropic immune cytokine secreted by activated TH2 cells. It has many biological roles, including the regulation in humoral and adaptive immunity, the inhibition of bone resorption, the stimulation of activated B-cell and T-cell proliferation, and initiation of allergic responses. Moreover, IL-4 induces a positive feedback loop by stimulating differentiation of naive helper T cells, or Th0 cells, to Th2 cells which in turn secrete more IL-4. Increased IL-4 production and overproduction of IL-4R are found in many cancers. IL-4 has a significant effect on tumor progression by increasing apoptosis resistance via tumor growth enhancement. IL-4 may also play a part in the immune dysregulation that occurs in most HIV-1 infected patients.
| Technical Datasheet: | View |
| Protein: | IL-4 |
| Research Area: | Cell Biology, Immunology, Neuroinflammation, Neuroscience |
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