![Anti-Mouse CD4 [GK1.5] In Vivo Antibody - Ultra Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/214396/569402/anti-mouse-cd4-gk1.5-in-vivo-antibody-ultra-low-endotoxin__92374.1770912415.jpg?c=2?imbypass=on "Anti-Mouse CD4 [GK1.5] In Vivo Antibody - Ultra Low Endotoxin")
Description
Anti-Mouse CD4 [GK1.5] In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Mouse CD4 [GK1.5] In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody tailored for in vivo applications. This antibody targets the CD4 protein, essential in immune response, making it ideal for research in immunology and related fields. With a Rat IgG2b isotype, it ensures high purity and ultra-low endotoxin levels (<0.002 EU/μg), perfect for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy.
Enhance your research with this reliable and versatile antibody. CD4 is a glycoprotein found on the surface of immune cells such as T-helper cells, monocytes, macrophages, and dendritic cells. It plays a significant role in the immune system by assisting the activation of T-cells through interaction with major histocompatibility complex (MHC) class II molecules.
| Product Name: | Anti-Mouse CD4 [GK1.5] In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0061 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | GK1.5 |
| Protein: | CD4 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | L3T4, T4 |
| Isotype: | Rat IgG2b κ |
| Reactivity: | Mouse |
| Immunogen: | Mouse CTL clone V4 |
| Applications: | B, Costim, CyTOF®, Depletion, FA, FC, IHC, IP |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, Costim, CyTOF®, Depletion, FA, FC, IHC, IP |
| Recommended Usage: | CyTOF®FC The suggested concentration for this GK1.5 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Rat Anti-Mouse CD4 (Clone GK1.5) recognizes an epitope on Mouse CD4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody was also pathogen tested and third-party certified by IDEXX BioReseach to meet the lowest mycoplasma specification and free of any viral pathogens of concern. |
| Antigen Distribution: | The CD4/L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines. |
| Immunogen: | Mouse CTL clone V4 |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.6
| Technical Datasheet: | View |
| Protein: | CD4 |
| Ligand/Receptor: | MHC class II molecule |
| Research Area: | Immunology |
| Rat IgG2b Isotype Control | |
|---|---|
| Clone | 1-2 |
| Isotype | Rat IgG2b kappa |
| Endotoxin Level | Ultra Low Endotoxin |
![Anti-Mouse CD4 [GK1.5] In Vivo Antibody - Ultra Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/608x608/products/214396/569402/anti-mouse-cd4-gk1.5-in-vivo-antibody-ultra-low-endotoxin__92374.1770912415.jpg?c=2 "Anti-Mouse CD4 [GK1.5] In Vivo Antibody - Ultra Low Endotoxin")
![Anti-Mouse CD4 [GK1.5] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/214395/569396/anti-mouse-cd4-gk1.5-in-vivo-antibody-low-endotoxin__43319.1774213559.jpg?c=2 "Anti-Mouse CD4 [GK1.5] In Vivo Antibody - Low Endotoxin")
