Anti-Mouse/Human IL-5 In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Mouse/Human IL-5 In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody engineered for in vivo applications. This antibody precisely targets the IL-5 protein, a crucial cytokine involved in the immune response, particularly in the regulation of eosinophil activities, making it an excellent tool for research in immunology, allergy, and related fields. With a mouse IgG2a isotype, it guarantees high purity and ultra-low endotoxin levels (<1.0 EU/mg), ensuring reliability and minimal immunogenicity in your experiments. This antibody is ideal for applications such as ELISA, flow cytometry, and functional assays in immunological studies.
It is available in various sizes and formulated in phosphate-buffered saline for optimal stability and performance. Elevate your research with the dependable and versatile Anti-Mouse/Human IL-5 In Vivo Antibody from Assay Genie. IL-5, or Interleukin 5, is a significant cytokine that plays a vital role in the immune system by promoting the growth and differentiation of eosinophils, a type of white blood cell involved in allergic reactions and asthma. By specifically targeting IL-5, this antibody aids in the detailed study of eosinophil-mediated responses and the development of therapeutic interventions for allergic diseases.
Product Name:
Anti-Mouse/Human IL-5 In Vivo Antibody - Ultra Low Endotoxin
Product Code:
IVMB0307
Size:
1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:
TRFK5
Protein:
IL-5
Product Type:
Monoclonal Antibody
Synonyms:
BCDFII,Interleukin-5, IL5, B cell differentiation factor 1, TRF, EDF
Isotype:
IgG1
Reactivity:
Mouse
Applications:
CyTOF®, Depletion, ELISA Cap, ELISPOT, FA, FC, IHC FFPE, In Vivo, N, WB
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
≤ 0.5 EU/mg as determined by the LAL method
Purity:
≥98% monomer by analytical SEC â‹… >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
CyTOF®, Depletion, ELISA Cap, ELISPOT, FA, FC, IHC FFPE, In Vivo, N, WB
Reactivity:
Mouse
Host Species:
Rat
Specificity:
Clone TRFK5 recognizes mouse/human IL-5.
Immunogen:
Partially-purified T cell clone supernatant
Concentration:
≥ 5.0 mg/ml
Endotoxin Level:
≤ 0.5 EU/mg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥98% monomer by analytical SEC â‹… >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
IL-5 is a 26 kD TH2 cytokine and a homodimeric glycoprotein that is part of the hematopoietic family. IL-5 stimulates B cell growth as well as increases Ig secretion. It is the main factor that promotes the terminal differentiation of eosinophil progenitors and enhances the effector capacity of mature eosinophils. IL-5 is a major regulator of eosinophil accumulation in tissues. In fact, in Hodgkin lymphoma, the characteristically observed eosinophilia is suspected to result from an increased production of IL-5. IL-5 may also be the cause of several allergic diseases including asthma, where it is thought that eosinophils have a significant role in the disease pathology. There are several mAbs currently in use that target IL-5 for the treatment of severe eosinophilic asthma.
Protein:
IL-5
Pathogen Testing:
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Assay Genie's Purified Functional Antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
