The Anti-Phospho Beclin-1 (Ser15) Antibody (CABP1252) is a valuable tool for researchers studying the role of Beclin-1 in autophagy and cell signaling pathways. Phosphorylation at Ser15 of Beclin-1 is known to regulate its activity, making this antibody essential for studying the phosphorylation status of Beclin-1 in various cellular processes.This antibody, raised in rabbits, exhibits high specificity and sensitivity in detecting phosphorylated Beclin-1 at Ser15 in human samples. It has been validated for use in Western blot applications, allowing for precise detection and quantification of phosphorylated Beclin-1 in cell lysates. Its ability to specifically bind to phospho-Beclin-1 at Ser15 makes it an ideal tool for researchers investigating autophagy, cancer, and other diseases where Beclin-1 phosphorylation plays a role.
The phosphorylation of Beclin-1 at Ser15 is a crucial event that regulates its interaction with other autophagy proteins and signaling molecules, contributing to the control of autophagic activity and cellular homeostasis. Research involving the phosphorylation status of Beclin-1 can provide valuable insights into the mechanisms that govern autophagy and its implications in disease pathology, paving the way for the development of targeted therapies for a variety of conditions.
Product Name:
Phospho-Beclin 1-Ser15 Rabbit Polyclonal Antibody
SKU:
CABP1252
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S15 of human Beclin 1 (NP_003757.1).
This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
8678
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-Beclin 1-Ser15 Rabbit pAb (CABP1252) at 1:500 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
