The Phospho-Beclin 1-Ser90 Antibody (CABP1254) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, raised against phosphorylated Ser90 of Beclin-1, is highly specific and sensitive, making it an ideal choice for Western blot and immunohistochemistry applications.Beclin-1 is known to be phosphorylated at Ser90 in response to various cellular stressors, such as nutrient deprivation and oxidative stress, leading to its activation and initiation of autophagy. The phosphorylation status of Beclin-1 at Ser90 is crucial for regulating autophagic activity and maintaining cellular homeostasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-Beclin 1-Ser90 Antibody
SKU:
CABP1254
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
MMST E
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
8678
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from C6 cells using Phospho-Beclin 1-Ser90 Rabbit pAb (CABP1254) at 1:400 dilution. C6 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
