Anti-Phospho-Paxillin-Y31 Antibody

Product Name:	Phospho-Paxillin-Y31 Rabbit pAb
Product Code:	CABP0206
Size:	50uL, 100uL
Synonyms:	Paxillin, PXN, paxillin
Applications:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	A synthetic phosphorylated peptide around Y31 of human Paxillin (NP_001074324.1).

Applications:	WB
Recommended Dilutions:	WB 1:500 - 1:2000
Reactivity:	Human

Immunogen:	A synthetic phosphorylated peptide around Y31 of human Paxillin (NP_001074324.1).
Purification Method:	Affinity purification
Storage:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	5829
Uniprot:	P49023
Cellular Location:	Cell junction, Cytoplasm, cell cortex, cytoskeleton, focal adhesion
Calculated MW:	46kDa/60kDa/64kDa/66kDa
Observed MW:	Refer to figures

UniProt Protein Function:	PXN: a multi-domain cytoskeletal protein involved in actin-membrane attachment that localizes primarily to focal adhesion sites to the extracellular matrix. Phosphorylated by focal adhesion kinase (FAK) and is a component of integrin signaling. Its phosphorylation provides docking sites for recruitment of signaling molecules to focal adhesions. Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the Crk SH2 domain. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds DDEF2. Interacts with unphosphorylated ITGA4. Interacts with RNF5.Three alternatively-spliced isoforms have been described. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase.
UniProt Protein Details:	Protein type:Adaptor/scaffold; Cytoskeletal; Motility/polarity/chemotaxis; Cell adhesion Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: nucleoplasm; microtubule associated complex; focal adhesion; lamellipodium; cytoplasm; stress fiber; plasma membrane; cell cortex; cytosol Molecular Function:integrin binding; protein binding; zinc ion binding; beta-catenin binding; BH4 domain binding; protein kinase binding; vinculin binding Biological Process: integrin-mediated signaling pathway; lamellipodium biogenesis; epidermal growth factor receptor signaling pathway; focal adhesion formation; regulation of cell shape; peptidyl-tyrosine phosphorylation; branching morphogenesis of a tube; muscle contraction; activation of MAPK activity; transforming growth factor beta receptor signaling pathway; signal complex assembly; cytoskeleton organization and biogenesis; vascular endothelial growth factor receptor signaling pathway; signal transduction; cell adhesion
NCBI Summary:	This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]
UniProt Code:	P49023
NCBI GenInfo Identifier:	317373486
NCBI Gene ID:	5829
NCBI Accession:	P49023.3
UniProt Secondary Accession:	P49023,O14970, O14971, O60360, Q5HYA4, B2RAI3, B7ZMB4
UniProt Related Accession:	P49023
Molecular Weight:	68kDa
NCBI Full Name:	Paxillin
NCBI Synonym Full Names:	paxillin
NCBI Official Symbol:	PXN
NCBI Protein Information:	paxillin
UniProt Protein Name:	Paxillin
Protein Family:	Paxillin
UniProt Gene Name:	PXN
UniProt Entry Name:	PAXI_HUMAN