The PLC beta 3 (PLCB3) Monoclonal Antibody (CAB19738) is a high-quality antibody developed for reliable detection and analysis of target proteins. PLCB3 is a member of the phospholipase C (PLC) family, which plays a key role in intracellular signaling by catalyzing the hydrolysis of phosphatidylinositol 4,5-bisphosphate into inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).This antibody, produced in rabbits, is specifically designed to target PLCB3 in human samples and has been validated for use in Western blot applications. By binding to PLCB3, researchers can detect and analyze the protein in various cell types, providing valuable insights into its function in normal cellular processes and disease states.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
PLC beta 3 (PLCB3) Monoclonal Antibody
SKU:
CAB19738
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC2269
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SMDCD, PLC beta 3 (PLCB3)
Positive Sample:
HeLa, OVCAR3
Cellular Localization:
Cytosol, Nucleus, Postsynaptic Cytosol.
Calculated MW:
139kDa
Observed MW:
150kDa
This gene encodes a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
5331
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PLC beta 3 (PLCB30) Rabbit mAb (CAB19738) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
