The Pumilio 2 Monoclonal Antibody (CAB19783) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a protein that belongs to a family of RNA-binding proteins. The encoded protein functions as a translational repressor during embryonic development and cell differentiation. This protein is also thought to be a positive regulator of cell proliferation in adipose-derived stem cells. Alternate splicing results in multiple transcript variants. RRID Gene ID 23369 Swiss Prot Synonym PUMH2; PUML2; Pumilio 2
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Pumilio 2 Monoclonal Antibody
SKU:
CAB19783
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2308
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PUMH2, PUML2, Pumilio 2
Positive Sample:
PC-3, Mouse testis, Rat testis
Cellular Localization:
Cytoplasm, Cytoplasmic Stress Granule, Cytosol, Nuclear Membrane, Perinuclear Region Of Cytoplasm.
Calculated MW:
114kDa
Observed MW:
110-125kDa
This gene encodes a protein that belongs to a family of RNA-binding proteins. The encoded protein functions as a translational repressor during embryonic development and cell differentiation. This protein is also thought to be a positive regulator of cell proliferation in adipose-derived stem cells. Alternate splicing results in multiple transcript variants. RRID Gene ID 23369 Swiss Prot Synonym PUMH2; PUML2; Pumilio 2
Purification Method:
Affinity purification
Gene ID:
23369
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat testis, using Pumilio 2 Rabbit mAb (CAB19783) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of various lysates using Pumilio 2 Rabbit mAb (CAB19783) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
