The APRT Antibody (CAB13946) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the APRT protein, researchers can easily detect and analyze APRT expression in various cell types.APRT, or adenine phosphoribosyltransferase, plays a crucial role in the salvage pathway of purine metabolism, which is essential for DNA synthesis and energy production in cells. Dysregulation of APRT has been implicated in various diseases, including gout and kidney stones.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
APRT Antibody
SKU:
CAB13946
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AMP, APRTD, APRT
Positive Sample:
Rat lung
Cellular Localization:
Cytoplasm.
Calculated MW:
20kDa
Observed MW:
22kDa
Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
353
RRID
AB_2760799
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat lung, using APRT Rabbit pAb (CAB13946) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
Immunofluorescence analysis of C6 cells using APRT Rabbit pAb (CAB13946) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
