The ATIC Antibody (CAB5559) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, has high reactivity with human samples and is suitable for use in Western blot applications. By binding to the ATIC protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for studies in biochemistry, molecular biology, and cancer research.ATIC plays a crucial role in nucleotide metabolism, making it a potential target for cancer therapy and other diseases characterized by dysregulated cellular proliferation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
ATIC Antibody
SKU:
CAB5559
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PURH, AICAR, AICARFT, IMPCHASE, HEL-S-70p, ATIC
Positive Sample:
MCF7, SW480, NCI-H460, HepG2, SW620, HT-29, K-562
Cellular Localization:
Cytosol, Extracellular Exosome, Plasma Membrane.
Calculated MW:
65kDa
Observed MW:
62kDa
This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria.
Purification Method
Affinity purification
Gene ID
471
RRID
AB_2766339
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ATIC Rabbit pAb (CAB5559) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
