The Beclin-1 Polyclonal Antibody from AssayGenie is a powerful tool for researchers studying autophagy and cancer biology. This antibody, generated in rabbits, is highly specific for Beclin-1 and is validated for use in Western blotting, immunofluorescence, and immunohistochemistry applications. Beclin-1 is a key regulator of the autophagy process, playing a critical role in maintaining cellular homeostasis and protecting against various diseases, including cancer. Dysregulation of Beclin-1 has been implicated in the progression of cancer and other disorders, making it an important target for therapeutic intervention.
By targeting Beclin-1 with this high-quality polyclonal antibody, researchers can accurately detect and analyze Beclin-1 expression in cell lines and tissue samples, providing valuable insights into the role of autophagy in disease pathology. This antibody is an essential tool for advancing research in the fields of autophagy, cancer biology, and therapeutic development.
Product Name:
Beclin 1 Polyclonal Antibody
SKU:
CAB21695
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1).
This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
8678
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and Beclin 1 knockout (KO) 239T cells using [KO Validated] Beclin 1 Rabbit pAb (CAB21695) at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
![Western blot analysis of extracts from HeLa cells, using [KO Validated] Beclin 1 Rabbit pAb (CAB21695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer:3% nonfat dry milk in TBST.](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/222631/580515/A21695_1__65999.1701341694.jpg?c=2?imbypass=on "Western blot analysis of extracts from HeLa cells, using [KO Validated] Beclin 1 Rabbit pAb (CAB21695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer:3% nonfat dry milk in TBST.")
![Western blot analysis of extracts from HeLa cells, using [KO Validated] Beclin 1 Rabbit pAb (CAB21695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer:3% nonfat dry milk in TBST.](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/608x608/products/222631/580515/A21695_1__65999.1701341694.jpg?c=2 "Western blot analysis of extracts from HeLa cells, using [KO Validated] Beclin 1 Rabbit pAb (CAB21695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer:3% nonfat dry milk in TBST.")
