Description
| SKU: | RPCB2989 |
| Size: | 100 μg |
| Tag: | C-His-Avi |
| Reactivity: | SARS-COV-2 |
| Protein Description: | Biotinylated Recombinant SARS-COV-2 SARS-COV-2 Spike S1 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gln14-Arg683) of SARS-COV-2 SARS-COV-2 Spike S1 fused with His tag and Avi tag at the C-terminal. |
| Endotoxin: | < 1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Gene ID: | 43740568 |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
 | Biotinylated Recombinant SARS- COV-2 Spike S1 Protein was determined by Tris-Bis PAGE under reducing conditions. |
 | The purity of Biotinylated SARS- COV-2 Spike S1 is greater than 95% as determined by SEC-HPLC. |
 | Immobilized Biotinylated SARS- COV-2 Spike S1, Hisi Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA. |
