Description
CA125 Polyclonal Antibody (CAB24521)
The CA125 Polyclonal Antibody (CAB24521) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the CA125 protein, this antibody allows for the accurate detection and analysis of CA125 levels in various cell types, making it ideal for studies in cancer research and diagnostics.CA125 is a glycoprotein that is overexpressed in ovarian cancer cells, making it a useful marker for the early detection and monitoring of this deadly disease.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | CA125 Polyclonal Antibody |
| SKU: | CAB24521 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | GFTHW IPVPT SSTPG TSTVD LGSGT PSSLP SPTTA GPLLV PFTLN FTITN LKYEE DMHCP GSRKF NTTER VLQSL LGPMF KNTSV GPLYS GCRLT LLRSE KDGAA TGVDA ICTHR LDPKS PGVDR EQLYW ELSQL TNGIK ELGPY TLDRN SLYVN GFTHQ TSAPN TSTPG TSTVD LGTSG TPSSL PSPTS AGPLL VPFTL NFTIT NLQYE EDMHH PGSRK FNTTE RVLQG LLGPM FKNTS VGLLY SGCRL TLLRP EKNGA ATGM | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | MUC16, CA125, mucin-16 |
| Positive Sample: | OVCAR-3 |
| Cellular Localization: | Cell Membrane, Secreted, Single-Pass Type I Membrane Protein, Extracellular Space. |
| Calculated MW: | 1519kDa |
| Observed MW: | Refertofigures |
This gene encodes a protein that is a member of the mucin family. Mucins are high molecular weight, O-glycosylated proteins that play an important role in forming a protective mucous barrier, and are found on the apical surfaces of the epithelia. The encoded protein is a membrane-tethered mucin that contains an extracellular domain at its amino terminus, a large tandem repeat domain, and a transmembrane domain with a short cytoplasmic domain. The amino terminus is highly glycosylated, while the repeat region contains 156 amino acid repeats unit that are rich in serines, threonines, and prolines. Interspersed within the repeats are Sea urchin sperm protein Enterokinase and Agrin (SEA) modules, leucine-rich repeats and ankyrin (ANK) repeats. These regions together form the ectodomain, and there is a potential cleavage site found near an SEA module close to the transmembrane domain. This protein is thought to play a role in forming a barrier, protecting epithelial cells from pathogens. Products of this gene have been used as a marker for different cancers, with higher expression levels associated with poorer outcomes.
| Purification Method | Affinity purification |
| Gene ID | 94025 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates, using CA125 Rabbit pAb (CAB24521) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC):U-937 Exposure time: 20s. |
