Cathepsin B (CTSB) Inhibitor Screening Kit (Fluorometric) (BN00427)

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SKU:
BN00427
€714

Description

ELISA Kit Technical ManualMSDS

Cathepsin B (CTSB) Inhibitor Screening Kit (Fluorometric)

Cathepsin B (CTSB, EC 3.4.22.1) is a lysosomal cysteine proteinase that is suggested to participate in intracellular degradation and turnover of proteins. It has also been implicated in tumor invasion and metastasis. Assay Genie's Cathepsin B Inhibitor Screening Kit utilizes the ability of Cathepsin B to cleave the synthetic AFC based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin B-specific inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize the potential inhibitors of Cathepsin B.

Figure: Inhibition of Cathepsin B activity by CTSB Inhibitor. Assay was performed following kit protocol.

Key Information Description

Product SKU

BN00427

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 400/505 nm)

Applications

Screen/study/characterize potential inhibitors of Cathepsin B.

Features and Benefits

  • Simple & convenient
  • High-throughput adaptable assay to screen/study/characterize the potential inhibitors of Cathepsin B
  • Cathepsin B inhibitor, F-F-FMK is included in the kit

Kit Components

  • CTSB Reaction Buffer
  • CTSB Reagent
  • Cathepsin B (human)
  • CTSB Substrate, Ac-RR-AFC (10 mM)
  • CTSB Inhibitor (F-F-FMK, 1 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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