Cathepsin L Inhibitor Screening Kit (Fluorometric) (BN00441)

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SKU:
BN00441
€738

Description

ELISA Kit Technical ManualMSDS

Cathepsin L Inhibitor Screening Kit (Fluorometric)

Cathepsin L (CTSL, EC 3.4.22.15) is a lysosomal cysteine protease that is implicated in protein degradation, arthritis, apoptosis, and cancer. Assay Genie's Cathepsin L Inhibitor Screening Kit utilizes the ability of active Cathepsin L to cleave the synthetic AFC-based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin L inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Cathepsin L.

Figure: Inhibition of Cathepsin L activity by CTSL Inhibitor. Assay was performed following the kit protocol.

Key Information Description

Product SKU

BN00441

Size

100 assays

Detection Method

Fluorescence (Ex/Em 400/505 nm)

Applications

Screen/study/characterize potential inhibitors of Cathepsin L

Features and Benefits

  • Convenient & Rapid
  • High-throughput adaptable assay to screen/study/characterize the potential inhibitors of Cathepsin L
  • Cathepsin L inhibitor, FF-FMK is included in the kit

Kit Components

  • CTSL Assay Buffer
  • CTSL Reagent
  • DTT
  • Cathepsin L (human, 20 mU)
  • CTSL Substrate, Ac-FR-AFC (10 mM)
  • CTSL Inhibitor (FF-FMK, 1 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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