The CBX8 Antibody (CAB6222) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blotting applications.CBX8 is a crucial component of the polycomb repressive complex 1 (PRC1), which plays a key role in gene silencing and chromatin compaction. By targeting CBX8, researchers can investigate its function in various cellular processes, such as gene expression regulation, cell differentiation, and development.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CBX8 Antibody
SKU:
CAB6222
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PC3, RC1, CBX8
Positive Sample:
A-549, 293T, HeLa, MCF-7, Mouse thymus
Cellular Localization:
Nucleus.
Calculated MW:
43kDa
Observed MW:
50kDa
Enables methylated histone binding activity. Involved in negative regulation of transcription by RNA polymerase II. Located in chromatin and nucleoplasm. Part of PRC1 complex. Biomarker of esophagus squamous cell carcinoma and glioblastoma.
Purification Method
Affinity purification
Gene ID
57332
RRID
AB_2766831
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CBX8 Rabbit pAb (CAB6222) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Rat pancreas using CBX8 Rabbit pAb (CAB6222) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis using CBX8 Rabbit pAb (CAB6222) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
