The CCDC47 Antibody (CAB15871) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity for human samples and has been validated for use in applications such as Western blotting. By binding to the CCDC47 protein, this antibody enables precise detection and analysis in various cell types, making it ideal for investigations in cell biology, cancer research, and developmental biology.CCDC47, also known as Coiled-Coil Domain Containing 47, is implicated in various cellular processes, including cell cycle regulation and DNA repair.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CCDC47 Antibody
SKU:
CAB15871
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
THNS, GK001, MSTP041, CCDC47
Positive Sample:
LO2, OVCAR3, Mouse lung
Cellular Localization:
Membrane, Single-Pass Membrane Protein.
Calculated MW:
56kDa
Observed MW:
60kDa
Enables protein folding chaperone and ribosome binding activity. Involved in ERAD pathway; endoplasmic reticulum calcium ion homeostasis; and protein insertion into ER membrane. Located in endoplasmic reticulum. Is integral component of endoplasmic reticulum membrane.
Purification Method
Affinity purification
Gene ID
57003
RRID
AB_2763300
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CCDC47 Rabbit pAb (CAB15871) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Immunofluorescence analysis of C6 cells using CCDC47 Rabbit pAb (CAB15871) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using CCDC47 Rabbit pAb (CAB15871) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
