The CCL5 Polyclonal Antibody (CAB24848) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity for human samples and has been validated for use in Western blotting. By binding to the CCL5 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in immunology and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
CCL5 Polyclonal Antibody
SKU:
CAB24848
Size:
20μL, 100μL
Reactivity:
Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
SDTT PCCF AYLS LALP RAHV KEYF
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
C-C motif chemokine 5
Positive Sample:
Rat lung
Cellular Localization:
Secreted.
Calculated MW:
10kDa
Observed MW:
13kDa
This gene is one of several chemokine genes clustered on the q arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immune regulation and inflammatory processes. According to the arrangement of N-terminal cysteine residues in mature peptides, the superfamily is divided into four subfamilies. This chemotactic factor belongs to the CC subfamily and is a chemotactic agent for blood monocytes, memory T helper cells, and eosinophils. It causes basophils to release histamine and activate eosinophils. This cytokine is one of the main HIV inhibitors produced by CD8+cells. It is one of the natural ligands of chemokine receptor chemokine receptor 5 (CCR5), which inhibits in vitro replication of HIV-1 R5 strains using CCR5 as a co receptor. Optional splicing leads to multiple transcriptional variants encoding different isomers.
Purification Method
Affinity purification
Gene ID
81780
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat lung using CCL5 Rabbit pAb (CAB24848) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
