The CD4 Antibody (CAB0362) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it suitable for various applications, including Western blot analysis.CD4, also known as T-cell surface glycoprotein CD4, is essential for initiating immune responses by binding to major histocompatibility complex class II molecules on antigen-presenting cells. Its presence on helper T cells is crucial for coordinating immune responses and activating other immune cells.
This antibody is validated for use in WB, IF/ICC, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CD4 Antibody
SKU:
CAB0362
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
T4, IMD79, Leu-3, OKT4D, CD4mut, CD4
Positive Sample:
U-937
Cellular Localization:
Cell Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
51kDa
Observed MW:
51kDa
This gene encodes the CD4 membrane glycoprotein of T lymphocytes. The CD4 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules. The CD4 antigen is also a primary receptor for entry of the human immunodeficiency virus through interactions with the HIV Env gp120 subunit. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, granulocytes, as well as in various regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
Purification Method
Affinity purification
Gene ID
920
RRID
AB_2757146
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from U-937 cells, using CD4 Rabbit pAb (CAB0362) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of Rat thymus cells using CD4 Rabbit pAb (CAB0362) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Jurkat cells using CD4 Rabbit pAb (CAB0362) at dilution of 1:100. Blue: DAPI for nuclear staining.
