The C/EBPB Monoclonal Antibody (CAB19538) is a high-quality antibody developed for reliable detection and analysis of target proteins. This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. RRID AB_2862655 Gene ID 1051 Swiss Prot Synonym TCF5; IL6DBP; NF-IL6; C/EBP-beta; C/EBPB
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
C/EBPB Monoclonal Antibody
SKU:
CAB19538
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC0017
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:6000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TCF5, IL6DBP, NF-IL6, C/EBP-beta, C/EBPB
Positive Sample:
HeLa
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
36kDa
Observed MW:
46 kDa (LAP)/48 kDa (Full-length)
This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. RRID AB_2862655 Gene ID 1051 Swiss Prot Synonym TCF5; IL6DBP; NF-IL6; C/EBP-beta; C/EBPB
Purification Method:
Affinity purification
Gene ID:
1051
RRID:
AB_2862655
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using C/EBPB Rabbit mAb (CAB19538) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): TT. Exposure time: 20 s.
