Clathrin heavy chain Monoclonal Antibody (CAB4943)
The Clathrin heavy chain Monoclonal Antibody (CAB4943) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated from rabbit hosts, exhibits high specificity and reactivity towards the clathrin heavy chain protein in various species, including human samples.Clathrin is a key protein involved in the formation of coated vesicles, which play a critical role in the internalization and transport of molecules within cells. By targeting the clathrin heavy chain, this antibody enables the precise detection and visualization of clathrin-coated structures in cells, making it an essential reagent for studies in cell biology, neuroscience, and drug delivery research.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Clathrin heavy chain Monoclonal Antibody
SKU:
CAB4943
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1228
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.
Purification Method
Affinity purification
Gene ID
1213 8218
RRID
AB_2863395
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Clathrin heavy chain Rabbit mAb (CAB4943) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Clathrin heavy chain Rabbit mAb (CAB4943) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Clathrin heavy chain Rabbit mAb (CAB4943) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of HeLa cells using Clathrin heavy chain Rabbit mAb (CAB4943, dilution 1:100) (Red). The cells were counterstained with α-Tubulin Rabbit mAb (AC049, dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
