The Cullin 2 Antibody (CAB5308) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By targeting the Cullin-2 protein, this antibody enables researchers to detect and analyze its expression in various cell types, making it an essential tool for studies in cell biology, cancer research, and protein degradation pathways.Cullin-2 is a vital component of the Cullin-RING E3 ubiquitin ligase complex, which plays a crucial role in protein degradation and regulation of cellular processes.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Cullin 2 Antibody
SKU:
CAB5308
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CUL2, cullin-2, Cullin 2
Positive Sample:
PC-3, K-562, HL-60, 293T, OVCAR3
Cellular Localization:
Cytosol, Nucleolus, Nucleoplasm.
Calculated MW:
87kDa
Observed MW:
87kDa
Enables ubiquitin protein ligase binding activity. Predicted to be involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process and protein ubiquitination. Predicted to act upstream of or within protein catabolic process. Located in nucleoplasm. Part of Cul2-RING ubiquitin ligase complex.
Purification Method
Affinity purification
Gene ID
8453
RRID
AB_2766120
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cullin 2 Rabbit pAb (CAB5308) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of A-549 cells using Cullin 2 Rabbit pAb (CAB5308). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
