The Cullin 4A Antibody (CAB2882) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity for human samples and is validated for use in Western blotting applications.Cullin-4A is involved in the regulation of protein turnover and plays a critical role in various cellular processes, including cell cycle control, DNA replication, and DNA repair. Dysregulation of Cullin-4A has been implicated in the development of cancer and other diseases, making it a promising target for therapeutic intervention.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cullin 4A Antibody
SKU:
CAB2882
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF/ICC
1:50 - 1:100
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CUL4A, cullin-4A, Cullin 4A
Positive Sample:
K-562, BT-474, HeLa, Mouse testis
Cellular Localization:
Nucleoplasm, Nucleus.
Calculated MW:
88kDa
Observed MW:
80kDa
CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins (Liu et al., 2009 [PubMed 19481525]).
Purification Method
Affinity purification
Gene ID
8451
RRID
AB_2764702
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Cullin 4A Rabbit pAb (CAB2882) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg Cullin 4A antibody (CAB2882). Western blot was performed from the immunoprecipitate using Cullin 4A antibody (CAB2882) at a dilution of 1:1000.
