The DGKA Antibody (CAB13969) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been extensively validated for use in Western blot applications.DGKA, also known as diacylglycerol kinase alpha, plays a crucial role in cellular processes such as lipid signaling, membrane trafficking, and cell growth. Dysregulation of DGKA has been implicated in various diseases, including cancer, diabetes, and neurological disorders.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DGKA Antibody
SKU:
CAB13969
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DAGK, DAGK1, DGK-alpha, DGKA
Positive Sample:
HeLa, Jurkat, Molt-4
Cellular Localization:
Cytosol, Plasma Membrane.
Calculated MW:
83kDa
Observed MW:
80kDa
The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene.
Purification Method
Affinity purification
Gene ID
1606
RRID
AB_2760823
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using DGKA Rabbit pAb (CAB13969) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
