The DKK1 Antibody (CAB2562) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By specifically binding to DKK1, this antibody enables accurate detection and analysis of DKK1 protein levels in various types of cells, making it ideal for investigations in developmental biology, cancer research, and bone metabolism studies.DKK1 is a key regulator of the Wnt signaling pathway, which plays a critical role in embryonic development, tissue regeneration, and cancer progression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DKK1 Antibody
SKU:
CAB2562
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SK, DKK-1, DKK1
Positive Sample:
HeLa, K-562, Mouse brain, Rat brain, Rat liver
Cellular Localization:
Secreted.
Calculated MW:
29kDa
Observed MW:
28-45kDa
This gene encodes a member of the dickkopf family of proteins. Members of this family are secreted proteins characterized by two cysteine-rich domains that mediate protein-protein interactions. The encoded protein binds to the LRP6 co-receptor and inhibits beta-catenin-dependent Wnt signaling. This gene plays a role in embryonic development and may be important in bone formation in adults. Elevated expression of this gene has been observed in numerous human cancers and this protein may promote proliferation, invasion and growth in cancer cell lines.
Purification Method
Affinity purification
Gene ID
22943
RRID
AB_2764450
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DKK1 Rabbit pAb (CAB2562) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
