The DNAJB12 Antibody (CAB14625) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. It binds specifically to DNAJB12, allowing for detection and analysis in a variety of cell types, making it ideal for research in cell biology and protein regulation.DNAJB12, also known as heat shock protein 40 (Hsp40), plays a crucial role in maintaining protein homeostasis and protecting against cellular stress.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DNAJB12 Antibody
SKU:
CAB14625
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DJ10, DNAJB12
Positive Sample:
293T, HT-29, BxPC-3, Mouse kidney, Mouse liver, Rat spleen
Cellular Localization:
Membrane, Single-Pass Membrane Protein.
Calculated MW:
42kDa
Observed MW:
41kDa
DNAJB12 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).
Purification Method
Affinity purification
Gene ID
54788
RRID
AB_2761499
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DNAJB12 Rabbit pAb (CAB14625) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
