The DNAL1 Antibody (CAB8267) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting the DNA Ligase I protein in human samples, making it an essential component for experiments utilizing Western blot techniques.DNA Ligase I is an enzyme critical for sealing breaks in DNA strands during replication and repair, ensuring the integrity of the genetic material.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DNAL1 Antibody
SKU:
CAB8267
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LC1, CILD16, C14orf168, DNAL1
Positive Sample:
Mouse testis
Cellular Localization:
Cytoplasm.
Calculated MW:
22kDa
Observed MW:
22kDa
This gene encodes an axonemal dynein light chain which functions as a component of the outer dynein arms complex. This complex acts as the molecular motor that provides the force to move cilia in an ATP-dependent manner. The encoded protein is expressed in tissues with motile cilia or flagella and may be involved in the movement of sperm flagella. Alternate splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
83544
RRID
AB_2769195
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from mouse testis, using DNAL1 Rabbit pAb (CAB8267) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using DNAL1 Rabbit pAb (CAB8267) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
