The Prolyl hydroxylase PHD1 (EGLN2) Antibody (CAB2252) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.EGL Nine Homolog 2, also known as EglN2 or PHD1, is a member of the prolyl hydroxylase family and plays a key role in oxygen sensing and response. Dysregulation of EGL Nine Homolog 2 has been implicated in various diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Prolyl hydroxylase PHD1 (EGLN2) Antibody
SKU:
CAB2252
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
HeLa, A-549, HepG2, U-87MG, MCF-7, Mouse brain, Rat heart
Cellular Localization:
Nucleus.
Calculated MW:
44kDa
Observed MW:
43kDa
The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.
Purification Method
Affinity purification
Gene ID
112398
RRID
AB_2764253
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Prolyl hydroxylase PHD1 (EGLN2) Rabbit pAb (CAB2252) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunoprecipitation analysis of 150 μg extracts of HeLa cells using 3 μg Prolyl hydroxylase PHD1 (Prolyl hydroxylase PHD1 (EGLN2)) antibody (CAB2252). Western blot was performed from the immunoprecipitate using Prolyl hydroxylase PHD1 (Prolyl hydroxylase PHD1 (EGLN2)) antibody (CAB2252) at a dilution of 1:1000.
