The eIF4A3 Antibody (CAB4338) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it an excellent choice for Western blot applications. By targeting the eIF4A3 protein, this antibody enables the accurate detection and analysis of eIF4A3 levels in various cell types, offering insights into its role in gene expression and protein synthesis.eIF4A3 is a crucial RNA helicase that plays a vital role in RNA metabolism and translation initiation. Dysregulation of eIF4A3 has been implicated in various diseases, including cancer and neurological disorders, making it a promising target for therapeutic intervention.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
eIF4A3 Antibody
SKU:
CAB4338
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
A-431, Jurkat, HeLa, A-549, NIH/3T3, Mouse spleen, Rat kidney
Cellular Localization:
Cytoplasm, Nucleus, Nucleus Speckle.
Calculated MW:
47kDa
Observed MW:
47kDa
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.
Purification Method
Affinity purification
Gene ID
9775
RRID
AB_2765633
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using eIF4A3 Rabbit pAb (CAB4338) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
