Anti-ETV1 Antibody (CAB13303)

Antibody Name:	Anti-ETV1 Antibody
Antibody SKU:	CAB13303
Antibody Size:	20uL, 50uL, 100uL
Application:	IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 80-300 of human ETV1 (NP_004947.2).

Application:	IHC IF
Recommended Dilution:	IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 80-300 of human ETV1 (NP_004947.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AFHG LPLK IKKE PHSP CSEI SSAC SQEQ PFKF SYGE KCLY NVSA YDQK PQVG MRPS NPPT PSST PVSP LHHA SPNS THTP KPDR AFPA HLPP SQSI PDSS YPMD HRFR RQLS EPCN SFPP LPTM PREG RPMY QRQM SEPN IPFP PQGF KQEY HDPV YEHN TMVG SAAS QSFP PPLM IKQE PRDF AYDS EVPS CHSI YMRQ EGFL AHPS RTEG CMFE KGPR Q
Gene ID:	2115
Uniprot:	P50549
Cellular Location:	Nucleus
Calculated MW:	43kDa/48kDa/50kDa/52kDa/53kDa/55kDa
Observed MW:

Synonyms:

ETV1, ER81

Background:

This gene encodes a member of the ETS (E twenty-six) family of transcription factors. The ETS proteins regulate many target genes that modulate biological processes like cell growth, angiogenesis, migration, proliferation and differentiation. All ETS proteins contain an ETS DNA-binding domain that binds to DNA sequences containing the consensus 5'-CGGA[AT]-3'. The protein encoded by this gene contains a conserved short acidic transactivation domain (TAD) in the N-terminal region, in addition to the ETS DNA-binding domain in the C-terminal region. This gene is involved in chromosomal translocations, which result in multiple fusion proteins including EWS-ETV1 in Ewing sarcoma and at least 10 ETV1 partners (see PMID: 19657377, Table 1) in prostate cancer. In addition to chromosomal rearrangement, this gene is overexpressed in prostate cancer, melanoma and gastrointestinal stromal tumor. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

UniProt Protein Function:	ETV1: Transcriptional activator that binds to DNA sequences containing the consensus pentanucleotide 5'-CGGA[AT]-3'. Defects in ETV1 are a cause of Ewing sarcoma (ES). A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. A chromosomal aberration involving ETV1 is found in patients with Erwing sarcoma. Translocation t(7;22)(p22;q12) with EWSR1. Belongs to the ETS family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Oncoprotein; Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 7p21.3 Cellular Component: nucleus Molecular Function:protein binding; transcription factor activity Biological Process: peripheral nervous system neuron development; positive regulation of transcription from RNA polymerase II promoter
NCBI Summary:	This gene encodes a member of the ETS (E twenty-six) family of transcription factors. The ETS proteins regulate many target genes that modulate biological processes like cell growth, angiogenesis, migration, proliferation and differentiation. All ETS proteins contain an ETS DNA-binding domain that binds to DNA sequences containing the consensus 5'-CGGA[AT]-3'. The protein encoded by this gene contains a conserved short acidic transactivation domain (TAD) in the N-terminal region, in addition to the ETS DNA-binding domain in the C-terminal region. This gene is involved in chromosomal translocations, which result in multiple fusion proteins including EWS-ETV1 in Ewing sarcoma and at least 10 ETV1 partners (see PMID: 19657377, Table 1) in prostate cancer. In addition to chromosomal rearrangement, this gene is overexpressed in prostate cancer, melanoma and gastrointestinal stromal tumor. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2016]
UniProt Code:	P50549
NCBI GenInfo Identifier:	12643411
NCBI Gene ID:	2115
NCBI Accession:	P50549.2
UniProt Secondary Accession:	P50549,O75849, Q4KMQ6, Q59GA7, A4D118, B2R768, B7Z2I4 B7Z618, B7Z9P2, C9JT37, E9PHB1, F5GXR2,
UniProt Related Accession:	P50549
Molecular Weight:	50,217 Da
NCBI Full Name:	ETS translocation variant 1
NCBI Synonym Full Names:	ETS variant 1
NCBI Official Symbol:	ETV1
NCBI Official Synonym Symbols:	ER81
NCBI Protein Information:	ETS translocation variant 1
UniProt Protein Name:	ETS translocation variant 1
UniProt Synonym Protein Names:	Ets-related protein 81
Protein Family:	ETS translocation
UniProt Gene Name:	ETV1
UniProt Entry Name:	ETV1_HUMAN

	Immunohistochemistry of paraffin-embedded rat pancreas using ETV1 antibody (CAB13303) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using ETV1 antibody (CAB13303) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse pancreas using ETV1 antibody (CAB13303) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using ETV1 antibody (CAB13303).

Antibodies	ELISA Kits
Anti-ETV1 Antibody (CAB7184)	ER81 Colorimetric Cell-Based ELISA
ETV1 Antibody (PACO06471)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)