The Exportin 5 (XPO5) Antibody (CAB6790) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the karyopherin family that is required for the transport of small RNAs and double-stranded RNA-binding proteins from the nucleus to the cytoplasm. The encoded protein translocates cargo through the nuclear pore complex in a RanGTP-dependent process.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Exportin 5 (XPO5) Antibody
SKU:
CAB6790
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCIPELISA
Recommended Dilution:
WB
1:2000 - 1:10000
IF/ICC
1:100 - 1:500
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
exp5, Exportin 5 (XPO5)
Positive Sample:
K-562
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
136kDa
Observed MW:
136kDa
This gene encodes a member of the karyopherin family that is required for the transport of small RNAs and double-stranded RNA-binding proteins from the nucleus to the cytoplasm. The encoded protein translocates cargo through the nuclear pore complex in a RanGTP-dependent process.
Purification Method
Affinity purification
Gene ID
57510
RRID
AB_2767373
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Exportin 5 (XPO5) Rabbit pAb (CAB6790) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of MCF7 cells using Exportin 5 (XPO5) Rabbit pAb (CAB6790).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.
Immunofluorescence analysis of PC-12 cells using Exportin 5 (XPO5) Rabbit pAb (CAB6790) at dilution of 1:500 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 1 μg Exportin 5 (Exportin 5 (XPO5)) antibody (CAB6790). Western blot was performed from the immunoprecipitate using Exportin 5 (Exportin 5 (XPO5)) antibody (CAB6790) at a dilution of 1:1000.
