Description
FBXW5 Antibody (CAB9345)
The FBXW5 Antibody (CAB9345) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications.FBXW5 is part of the SCF (Skp1-Cullin1-F-box) ubiquitin ligase complex, which targets specific proteins for degradation by the proteasome. By targeting FBXW5, researchers can investigate its role in controlling the levels of key proteins within the cell, shedding light on its impact on various cellular functions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | FBXW5 Antibody |
| SKU: | CAB9345 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | VRNK PYDV FGCW LTET SLIS GNLH RIGD ITSC SVLW LNNA FQDV ESEN VNVV KRLF KIQN LNAS TVRT VMVA DCSR FDSP DLLL EAGD PATS PCRI FDLG SDNE EVVA GPAP AHAK EGLR HFLD RVLE GRAQ PQLS ERML ETKV AELL AQGH TKPP ERSA TGAK SKYL IFTT GCLT YSPH QIGI KQIL PHQM TTAG PVLG EGRG SDAF FDAL DHVI DIHG HIIG MGLS PDNR YLYV NSRA WPNG AVVA DPMQ PPPI AEEI DLLV FDLK TMRE VRRA LRAH RAYT PNDE CFFI FLDV SRDF VASG AEDR HGYI WDRH YNIC LARL RHED VVNS VVFS PQEQ ELLL TASD DATI KAWR SPRT MRVL QAPR PRPR TFFS WLAS QRR | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | Fbw5, FBXW5 |
| Positive Sample: | A-549 |
| Cellular Localization: | Cytoplasm. |
| Calculated MW: | 64kDa |
| Observed MW: | 72kDa |
This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene contains WD-40 domains, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene, however, they were found to be nonsense-mediated mRNA decay (NMD) candidates, hence not represented.
| Purification Method | Affinity purification |
| Gene ID | 54461 |
| RRID | AB_2769439 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from A-549 cells, using FBXW5 Rabbit pAb (CAB9345) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s. |
