Description
FOXO1 (Phospho-Ser256)Transcription Factor Activity Assay
The FOXO1 Phospho-Ser256 Transcription Factor Activity Assay Kit is a highly sensitive and specific assay designed for the accurate detection of FOXO1 activity in cell lysates. By targeting the phosphorylation of Ser256 on FOXO1, this kit allows for the measurement of transcription factor activity, providing valuable insights into cellular signaling pathways and gene regulation.FOXO1 is a key transcription factor that plays a critical role in diverse biological processes, including cell growth, metabolism, and stress response.
Dysregulation of FOXO1 activity has been implicated in various diseases, such as cancer, diabetes, and cardiovascular disorders, making it a promising target for therapeutic intervention.With its reliable performance and easy-to-use format, the FOXO1 Phospho-Ser256 Transcription Factor Activity Assay Kit is an essential tool for researchers studying FOXO1 biology and developing novel treatments for FOXO1-associated diseases.
| Product Name: | FOXO1 (Phospho-Ser256)Transcription Factor Activity Assay |
| Product Code: | TFAB00026 |
| Target: | FOXO1 (Phospho-Ser256) |
| Synonyms: | Forkhead box protein O1A, Forkhead in rhabdomyosarcoma |
| Reactivity: | Human, Mouse |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie FOXO1 (Phospho-Ser256) transcription factor activity assay allows for the detection and qualitative analysis of FOXO1 (Phospho-Ser256) active transcription factors in eukaryotic nuclear or cell lysates.
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | FOXO1A: a transcription factor of the forkhead family. A central regulator of metabolism in several cell types. May play an important role in myogenic growth and differentiation, and in the regulation of lipolysis in adipocytes by controlling the expression of adipose triglyceride lipase (ATGL), the rate-limiting lipolytic enzyme. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. Contains 1 fork-head domain. |
| UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; Oncoprotein; DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 13q14.1 Cellular Component: cytoplasm; cytosol; mitochondrion; nucleoplasm; nucleus Molecular Function:beta-catenin binding; chromatin binding; protein binding; protein phosphatase 2A binding; sequence-specific DNA binding; ubiquitin protein ligase binding Biological Process: anatomical structure morphogenesis; apoptosis; autophagy; blood vessel development; cell differentiation; cell glucose homeostasis; cellular response to insulin stimulus; cellular response to starvation; endocrine pancreas development; epidermal growth factor receptor signaling pathway; fat cell differentiation; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; negative regulation of apoptosis; negative regulation of fat cell differentiation; negative regulation of stress-activated MAPK cascade; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of apoptosis; positive regulation of autophagy; positive regulation of gluconeogenesis; positive regulation of protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid acetylation; response to DNA damage stimulus; thermoregulation; transcription, DNA-dependent Disease: Rhabdomyosarcoma 2 |
| NCBI Summary: | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q12778 |
| NCBI GenInfo Identifier: | 116241368 |
| NCBI Gene ID: | 2308 |
| NCBI Accession: | Q12778.2 |
| UniProt Secondary Accession: | Q12778,O43523, Q5VYC7, Q6NSK6, |
| UniProt Related Accession: | Q12778 |
| Molecular Weight: | |
| NCBI Full Name: | Forkhead box protein O1 |
| NCBI Synonym Full Names: | forkhead box O1 |
| NCBI Official Symbol: | FOXO1Â Â |
| NCBI Official Synonym Symbols: | FKH1; FKHR; FOXO1AÂ Â |
| NCBI Protein Information: | forkhead box protein O1 |
| UniProt Protein Name: | Forkhead box protein O1 |
| UniProt Synonym Protein Names: | Forkhead box protein O1A; Forkhead in rhabdomyosarcoma |
| Protein Family: | Foxo1-corepressor |
| UniProt Gene Name: | FOXO1Â Â |
| UniProt Entry Name: | FOXO1_HUMAN |
