Anti-GAD1 Antibody (CAB2938)

Antibody Name:	Anti-GAD1 Antibody
Antibody SKU:	CAB2938
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human GAD1 (NP_000808.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SKOV3, Jurkat, Mouse pancreas

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human GAD1 (NP_000808.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASS TPSS SATS SNAG ADPN TTNL RPTT YDTW CGVA HGCT RKLG LKIC GFLQ RTNS LEEK SRLV SAFK ERQS SKNL LSCE NSDR DARF RRTE TDFS NLFA RDLL PAKN GEEQ TVQF LLEV VDIL LNYV RKTF DRST KVLD FHHP HQLL EGME GFNL ELSD HPES LEQI LVDC RDTL KYGV RTGH PRFF NQLS TGLD IIGL
Gene ID:	2571
Uniprot:	Q99259
Cellular Location:
Calculated MW:	25kDa/47kDa/66kDa
Observed MW:	67kDa

Synonyms:

GAD1, CPSQ1, GAD, SCP

Background:

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

UniProt Protein Function:	GAD67: Catalyzes the production of GABA. Homodimer. Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. Belongs to the group II decarboxylase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 4.1.1.15; Other Amino Acids Metabolism - beta-alanine; Other Amino Acids Metabolism - taurine and hypotaurine; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; Lyase Chromosomal Location of Human Ortholog: 2q31 Cellular Component: plasma membrane; intracellular; vesicle membrane Molecular Function:protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; protein N-terminus binding; pyridoxal phosphate binding Biological Process: response to drug; glutamate catabolic process; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; gamma-aminobutyric acid biosynthetic process; protein-pyridoxal-5-phosphate linkage; neurotransmitter biosynthetic process Disease: Cerebral Palsy, Spastic Quadriplegic, 1
NCBI Summary:	This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99259
NCBI GenInfo Identifier:	1352213
NCBI Gene ID:	2571
NCBI Accession:	Q99259.1
UniProt Secondary Accession:	Q99259,Q49AK1, Q53TQ7, Q9BU91, Q9UHH4,
UniProt Related Accession:	Q99259
Molecular Weight:	66,897 Da
NCBI Full Name:	Glutamate decarboxylase 1
NCBI Synonym Full Names:	glutamate decarboxylase 1 (brain, 67kDa)
NCBI Official Symbol:	GAD1
NCBI Official Synonym Symbols:	GAD; SCP; CPSQ1
NCBI Protein Information:	glutamate decarboxylase 1; GAD-67; 67 kDa glutamic acid decarboxylase; glutamate decarboxylase 67 kDa isoform
UniProt Protein Name:	Glutamate decarboxylase 1
UniProt Synonym Protein Names:	67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform
Protein Family:	Glutamate decarboxylase
UniProt Gene Name:	GAD1
UniProt Entry Name:	DCE1_HUMAN

	Western blot analysis of extracts of various cell lines, using GAD1 antibody (CAB2938) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat brain using GAD1 Rabbit pAb (CAB2938) at dilution of 1:150 (40x lens).
	Immunofluorescence analysis of Rat eyeball using GAD1 antibody (CAB2938) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of Mouse eyeball using GAD1 antibody (CAB2938) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U2OS cells using GAD1 antibody (CAB2938) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
GAD1 Antibody (PACO03786)	Human GAD1 / Glutamate decarboxylase 1 ELISA Kit
	Human GAD1 (Glutamate Decarboxylase 1) CLIA Kit (HUES01242)
	Human Glutamate decarboxylase 1 (GAD1) ELISA Kit
	GAD1 Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)