The HRP-conjugated Donkey anti-Mouse IgG (H+L) (CABS033) is a high-quality antibody developed for reliable detection and analysis of target proteins. Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
This antibody is validated for use in WB, IHC-P applications and has demonstrated reactivity against Mouse samples.
Product Name:
HRP-conjugated Donkey anti-Mouse IgG (H+L)
SKU:
CABS033
Size:
100μL, 200μL
Reactivity:
Mouse
Conjugate:
HRP
Immunogen:
This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-P
Recommended Dilution:
WB
1:10000 - 1:20000
IHC-P
1:500 - 1:1000
Positive Sample:
Mouse control IgG, HeLa
Observed MW:
25kDa(Light chain),55kDa(Heavy chain)/36kDa
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method
Affinity purification
RRID
AB_2769847
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3.
Western blot analysis of recombinant Mouse control IgG Protein using HRP Donkey Anti-Mouse IgG (H+L)(CABS033) at 1:10000 dilution. Secondary antibody:HRP Donkey Anti-Mouse IgG (H+L)(CABS033) at 1:10000 dilution. Lysates/proteins: 100ng/50ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Western blot analysis of lysates from HeLa cells, using GAPDH antibody as the primary antibody. Secondary antibody:HRP Donkey Anti-Mouse IgG (H+L)(CABS033) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
