The HSD17B8 Antibody (CAB12947) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human samples and is validated for use in various applications, including Western blotting.HSD17B8 plays a crucial role in hormone regulation and lipid metabolism, making it a promising target for research related to hormone-dependent cancers, metabolic disorders, and reproductive health. By binding specifically to the HSD17B8 protein, this antibody allows for accurate detection and analysis in different cell types, providing researchers with valuable insights into its function and potential therapeutic applications.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HSD17B8 Antibody
SKU:
CAB12947
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
HepG2, BxPC-3, U-87MG, HeLa, Mouse liver, Mouse pancreas, Rat heart
Cellular Localization:
Mitochondrion Matrix.
Calculated MW:
27kDa
Observed MW:
27kDa
In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. The protein encoded by this gene is similar to Ke6 and is a member of the short-chain dehydrogenase superfamily. An alternatively spliced transcript of this gene has been detected, but the full-length nature of this variant has not been determined.
Purification Method
Affinity purification
Gene ID
7923
RRID
AB_2759793
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using HSD17B8 Rabbit pAb (CAB12947) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of U-2 OS cells using HSD17B8 Rabbit pAb (CAB12947) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
