The Human Acylated Ghrelin ELISA Kit is a cutting-edge tool for the precise measurement of acylated ghrelin levels in human biological samples such as serum and plasma. With its superior sensitivity and specificity, this kit delivers dependable and consistent results, making it the perfect solution for various research needs.Acylated ghrelin is a pivotal hormone known for its role in regulating appetite and energy metabolism. Its dysregulation has been linked to various metabolic disorders, making it a valuable biomarker for studying conditions like obesity, diabetes, and metabolic syndrome. By accurately quantifying acylated ghrelin levels, researchers can gain insights into the mechanisms underlying these conditions and explore potential therapeutic interventions.Overall, the Human Acylated Ghrelin ELISA Kit offers a reliable and convenient method for studying the physiological and pathological roles of acylated ghrelin, paving the way for advancements in metabolic research and potential therapeutic strategies.
Product Name:
Human A-GHRL (Acylated Ghrelin) ELISA Kit
SKU:
HUES02935
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
