Human ACT alpha2 (Actin Alpha 2, Smooth Muscle) CLIA Kit
The Human ACTA2 (Actin, Alpha 2, Smooth Muscle) CLIA Kit is specially designed for the precise measurement of alpha-2 smooth muscle actin levels in human samples, including serum, plasma, and cell culture supernatants. With its outstanding sensitivity and specificity, this kit delivers highly accurate and consistent results, making it an invaluable tool for various research purposes.Actin alpha-2 smooth muscle is a key protein involved in smooth muscle contraction and cell migration, playing a crucial role in various physiological processes such as wound healing, tissue remodeling, and blood vessel contraction.
Dysregulation of ACTA2 expression has been linked to conditions like hypertension, vascular diseases, and fibrotic disorders, highlighting its significance as a potential biomarker for disease detection and therapeutic development.
Product Name:
Human ACT alpha2 (Actin Alpha 2, Smooth Muscle) CLIA Kit
SKU:
HUES00193
Target:
Human ACT alpha2 (Actin Alpha 2, Smooth Muscle)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACTα2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACTα2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACTα2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ACTα2. You can calculate the concentration of Human ACTα2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
97-113
95-105
Average (%)
93
103
100
1:4
Range (%)
88-98
102-116
93-108
Average (%)
93
109
100
1:8
Range (%)
89-100
84-100
89-102
Average (%)
95
91
96
1:16
Range (%)
87-99
88-102
101-117
Average (%)
92
94
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
107
EDTA plasma (n=5)
86-100
92
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.92
3.64
13.64
2.05
3.64
13.76
Standard deviation
0.21
0.42
0.93
0.19
0.28
0.84
C V (%)
10.94
11.54
6.82
9.27
7.69
6.1
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ACTα2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ACTα2 in samples. No significant cross-reactivity or interference between Human ACTα2 and analogues was observed.
