The Human ADH (Antidiuretic Hormone) ELISA Kit is specifically designed for the precise quantification of ADH levels in human serum, plasma, and cell culture supernatants. This kit is renowned for its exceptional sensitivity and specificity, ensuring accurate and consistent results, thereby making it an invaluable tool for various research applications.ADH, also known as vasopressin, is a vital hormone that regulates water balance in the body by controlling the reabsorption of water in the kidneys. Imbalances in ADH levels can lead to disorders such as diabetes insipidus and syndrome of inappropriate antidiuretic hormone secretion (SIADH).
Therefore, measuring ADH levels is crucial for understanding and managing these conditions.With its advanced technology and user-friendly format, the Human ADH ELISA Kit is an essential resource for researchers studying fluid balance disorders, renal function, and other physiological processes influenced by ADH. Trust in this kit to deliver precise and reliable results that will advance your understanding of ADH-related pathways and enhance your research endeavors.
Product Name:
Human ADH (Antidiuretic Hormone) ELISA Kit
SKU:
HUES01508
Target:
Human ADH (Antidiuretic Hormone)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ADH. During the reaction, ADH in the sample or standard competes with a fixed amount of ADH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ADH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ADH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
88-99
84-96
Average (%)
98
93
90
1:4
Range (%)
85-95
90-106
99-112
Average (%)
90
97
107
1:8
Range (%)
87-101
87-97
97-111
Average (%)
93
92
105
1:16
Range (%)
87-99
90-105
93-106
Average (%)
94
96
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
96
EDTA plasma (n=5)
92-103
98
Cell culture media (n=5)
91-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.8
140.6
362.3
49.3
127.0
335.6
Standard deviation
2.7
5.8
12.0
3.1
6.2
11.1
C V (%)
5.9
4.13
3.31
6.29
4.88
3.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of ADH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes ADH in samples. No significant cross-reactivity or interference between ADH and analogues was observed.
