The Human Adiponectin Receptor 1 (ADIPOR1) ELISA Kit is specifically designed for the accurate measurement of ADIPOR1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.ADIPOR1 is a key receptor involved in the regulation of adiponectin, a hormone known for its anti-inflammatory and insulin-sensitizing properties. Dysregulation of adiponectin signaling through ADIPOR1 has been implicated in metabolic disorders such as obesity, type 2 diabetes, and cardiovascular diseases.
Therefore, this ELISA kit serves as a valuable tool for studying the role of ADIPOR1 in these conditions and potential therapeutic interventions.Overall, the Human Adiponectin Receptor 1 ELISA Kit provides researchers with a reliable means to quantify ADIPOR1 levels, furthering our understanding of adiponectin signaling pathways and its implications in various physiological and pathological processes.
Product Name:
Human Adiponectin Receptor 1 (ADIPOR1) ELISA Kit
Product Code:
HUDL00081
Size:
96 Assays
Target:
ADIPOR1
Synonyms:
ACDCR1, CGI45, PAQR1, TESBP1A, TESBP1-A, Progestin and AdipoQ Receptor Family Member I
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
