The Human AIF (Apoptosis-Inducing Factor) CLIA Kit is a reliable and efficient assay designed for the precise measurement of AIF levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it an invaluable tool for various research applications.AIF is a key protein involved in apoptosis, the process of programmed cell death. Dysregulation of AIF has been linked to various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
By accurately measuring AIF levels, researchers can gain important insights into the mechanisms of cell death and potentially identify new therapeutic targets for these conditions.Overall, the Human AIF CLIA Kit from Assaygenie provides researchers with a powerful tool to study apoptosis and its implications in disease pathogenesis. Its ease of use and reliability make it a valuable addition to any laboratory aiming to advance our understanding of cell death processes.
Product Name:
Human AIF (Apoptosis Inducing Factor) CLIA Kit
SKU:
HUES00344
Target:
Human AIF (Apoptosis Inducing Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AIF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AIF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AIF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AIF. You can calculate the concentration of Human AIF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-118
94-109
94-106
Average (%)
108
100
101
1:4
Range (%)
88-99
86-100
96-109
Average (%)
94
93
103
1:8
Range (%)
88-100
91-106
99-114
Average (%)
94
99
105
1:16
Range (%)
96-108
96-112
100-114
Average (%)
102
103
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
93-104
98
Cell culture media (n=5)
85-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.6
250.84
948.15
102.82
227.58
911.56
Standard deviation
9.39
18.44
89.6
12.53
25.17
55.7
C V (%)
9.82
7.35
9.45
12.19
11.06
6.11
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AIF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AIF in samples. No significant cross-reactivity or interference between Human AIF and analogues was observed.
