The human AlphaFP (Alpha-Fetoprotein) CLIA Kit is a highly reliable and efficient tool for the quantitative measurement of Alpha-Fetoprotein levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Alpha-Fetoprotein is a key biomarker that is commonly associated with various conditions such as liver cancer, hepatocellular carcinoma, and other liver diseases. By accurately measuring Alpha-Fetoprotein levels, researchers and clinicians can gain valuable insights into the progression and management of these conditions, ultimately leading to the development of targeted therapies and improved patient outcomes.
Overall, the human AlphaFP CLIA Kit is an indispensable tool for studying Alpha-Fetoprotein-related diseases and conducting groundbreaking research in the field of oncology and liver health. Its high performance and ease of use make it a valuable asset for any laboratory or research facility.
Product Name:
Human alphaFP (Alpha-Fetoprotein) CLIA Kit
SKU:
HUES00068
Target:
Human alphaFP (Alpha-Fetoprotein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human αFP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human αFP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human αFP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human αFP. You can calculate the concentration of Human αFP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-120
88-101
99-110
Average (%)
109
94
105
1:4
Range (%)
84-95
94-106
98-111
Average (%)
90
99
105
1:8
Range (%)
92-108
87-101
85-98
Average (%)
99
94
92
1:16
Range (%)
94-106
88-98
101-114
Average (%)
100
93
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
100
EDTA plasma (n=5)
99-112
106
Cell culture media (n=5)
87-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.1
2.72
7.51
1.0
2.89
7.49
Standard deviation
0.12
0.29
0.81
0.1
0.22
0.86
C V (%)
10.91
10.66
10.79
10.0
7.61
11.48
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human αFP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human αFP in samples. No significant cross-reactivity or interference between Human αFP and analogues was observed.
