Human ANG-R-Tie1 (Angiopoietin Receptor Tie1) CLIA Kit
The Human ANG-R (TIE1) Angiopoietin Receptor TIE1 CLIA Kit is a cutting-edge diagnostic tool designed for the precise measurement of Angiopoietin receptor TIE1 levels in human samples, including serum, plasma, and cell culture supernatants. This advanced kit offers unparalleled sensitivity and specificity, ensuring reliable and consistent results for a variety of research applications.Angiopoietin receptor TIE1 is a critical component in angiogenesis, playing a key role in blood vessel formation and cell proliferation.
Its involvement in various health conditions such as cancer, cardiovascular diseases, and neurodegenerative disorders underscores its significance as a valuable biomarker for both understanding these ailments and developing potential treatment strategies.Trust in the accuracy and precision of the Human ANG-R (TIE1) Angiopoietin Receptor TIE1 CLIA Kit to elevate your research to new heights and uncover groundbreaking insights into the complex mechanisms of angiogenesis and its impact on human health.
Product Name:
Human ANG-R-Tie1 (Angiopoietin Receptor Tie1) CLIA Kit
SKU:
HUES00241
Target:
Human ANG-R-Tie1 (Angiopoietin Receptor Tie1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG-R-Tie1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG-R-Tie1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANG-R-Tie1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ANG-R-Tie1. You can calculate the concentration of Human ANG-R-Tie1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
88-99
90-102
Average (%)
91
94
96
1:4
Range (%)
90-101
101-116
96-109
Average (%)
96
107
102
1:8
Range (%)
87-100
101-117
85-97
Average (%)
94
110
91
1:16
Range (%)
91-105
99-114
91-103
Average (%)
97
105
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-112
102
EDTA plasma (n=5)
88-102
94
Cell culture media (n=5)
94-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.69
189.38
745.08
102.31
176.92
678.08
Standard deviation
11.71
17.67
78.68
12.5
17.34
65.03
C V (%)
11.19
9.33
10.56
12.22
9.8
9.59
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ANG-R-Tie1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANG-R-Tie1 in samples. No significant cross-reactivity or interference between Human ANG-R-Tie1 and analogues was observed.
