The Human ANP (Atrial Natriuretic Peptide) ELISA Kit is specifically designed for the precise detection of atrial natriuretic peptide levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it perfect for a variety of research purposes.Atrial natriuretic peptide is a vital hormone involved in regulating blood pressure and fluid balance by promoting vasodilation and excretion of sodium and water. It plays a crucial role in conditions like heart failure, hypertension, and renal disorders, making it a key biomarker for understanding these diseases and developing potential treatments. Overall, the Human ANP ELISA Kit provides researchers with a valuable tool for studying the role of atrial natriuretic peptide in various physiological and pathological processes, paving the way for advancements in disease diagnosis and management.
Product Name:
Human ANP (Atrial Natriuretic Peptide) ELISA Kit
SKU:
HUES01727
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
