The Human APLN (Apelin) ELISA Kit is specifically designed for the precise measurement of apelin levels in human serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, guaranteeing accurate and consistent results, making it the perfect choice for various research purposes.Apelin is a key peptide that plays a vital role in regulating cardiovascular functions, fluid homeostasis, and energy metabolism. It is implicated in various physiological processes and diseases, including heart failure, obesity, and diabetes, making it a valuable biomarker for studying these conditions and developing targeted therapies. By using the Human APLN ELISA Kit, researchers can effectively measure apelin levels in biological samples, allowing for a better understanding of its role in disease pathogenesis and potential therapeutic interventions. Trust in this kit to enable groundbreaking discoveries in the field of apelin research.
Product Name:
Human APLN (Apelin) ELISA Kit
SKU:
HUES01662
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
