The Human APLN (Apelin) ELISA Kit is specifically designed for the precise measurement of apelin levels in human serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, guaranteeing accurate and consistent results, making it the perfect choice for various research purposes.Apelin is a key peptide that plays a vital role in regulating cardiovascular functions, fluid homeostasis, and energy metabolism. It is implicated in various physiological processes and diseases, including heart failure, obesity, and diabetes, making it a valuable biomarker for studying these conditions and developing targeted therapies.
By using the Human APLN ELISA Kit, researchers can effectively measure apelin levels in biological samples, allowing for a better understanding of its role in disease pathogenesis and potential therapeutic interventions. Trust in this kit to enable groundbreaking discoveries in the field of apelin research.
Product Name:
Human APLN (Apelin) ELISA Kit
SKU:
HUES01662
Target:
Human APLN (Apelin)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with APLN. During the reaction, APLN in the sample or standard competes with a fixed amount of APLN on the solid phase supporter for sites on the Biotinylated Detection Ab specific to APLN. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of APLN in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
98-114
88-102
Average (%)
100
105
94
1:4
Range (%)
85-97
89-101
99-115
Average (%)
90
95
105
1:8
Range (%)
87-101
90-104
97-111
Average (%)
94
97
102
1:16
Range (%)
84-94
92-103
97-112
Average (%)
89
97
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
90
EDTA plasma (n=5)
91-102
96
Cell culture media (n=5)
89-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
197.4
537.1
1359.9
204.3
499.7
1350.7
Standard deviation
11.1
31.7
50.3
11.0
26.0
56.7
C V (%)
5.62
5.9
3.7
5.38
5.2
4.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of APLN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes APLN in samples. No significant cross-reactivity or interference between APLN and analogues was observed.
