The Human APOA4 (Apolipoprotein A4) CLIA Kit is a powerful tool for the quantitative measurement of APOA4 levels in human samples such as serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, providing accurate and reproducible results for a variety of research applications.APOA4 is a critical protein involved in lipid metabolism and plays a key role in regulating cholesterol levels in the body. Dysregulation of APOA4 has been linked to various metabolic disorders, making it a valuable biomarker for investigating conditions such as obesity, diabetes, and cardiovascular diseases.
By accurately measuring APOA4 levels, researchers can gain valuable insights into these pathological processes and potentially identify novel therapeutic targets. Overall, the Human APOA4 CLIA Kit offers researchers a reliable and efficient means of studying the role of APOA4 in health and disease, making it an essential tool for advancing our understanding of metabolic disorders and informing future treatment strategies.
Product Name:
Human ApoA4 (Apolipoprotein A4) CLIA Kit
SKU:
HUES00330
Target:
Human ApoA4 (Apolipoprotein A4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.56 ng/mL
Detection range:
0.94-60 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ApoA4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoA4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoA4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ApoA4. You can calculate the concentration of Human ApoA4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
102-121
89-100
Average (%)
99
110
94
1:4
Range (%)
90-102
102-118
99-112
Average (%)
96
109
105
1:8
Range (%)
88-98
89-101
98-112
Average (%)
93
95
106
1:16
Range (%)
86-102
92-109
92-107
Average (%)
93
99
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
97
EDTA plasma (n=5)
85-101
92
Cell culture media (n=5)
87-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
3.0
7.19
22.99
3.13
7.63
22.8
Standard deviation
0.35
0.52
1.54
0.37
0.58
2.22
C V (%)
11.67
7.23
6.7
11.82
7.6
9.74
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ApoA4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoA4 in samples. No significant cross-reactivity or interference between Human ApoA4 and analogues was observed.
