Human APOA5 Recombinant Protein (RPPB0048)
- SKU:
- RPPB0048
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q6Q788
- Research Area:
- Cytokines
Description
| Product Name: | Human APOA5 Recombinant Protein |
| Product Code: | RPPB0048 |
| Size: | 10µg |
| Species: | Human |
| Target: | APOA5 |
| Synonyms: | Apolipoprotein A-V, Apo-AV, ApoA-V, Apolipoprotein A5, Regeneration-associated protein 3, APOA5, RAP3, APOAV. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | APOA5 protein solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSRKGFWDY FSQTSGDKGR VEQIHQQKMA REPATLKDSL EQDLNNMNKF LEKLRPLSGS EAPRLPQDPV GMRRQLQEEL EEVKARLQPY MAEAHELVGW NLEGLRQQLK PYTMDLMEQV ALRVQELQEQ LRVVGEDTKA QLLGGVDEAW ALLQGLQSRV VHHTGRFKEL FHPYAESLVS GIGRHVQELH RSVAPHAPAS PARLSRCVQV LSRKLTLKAK ALHARIQQNL DQLREELSRA FAGTGTEEGA GPDPQMLSEE VRQRLQAFRQ DTYLQIAAFT RAIDQETEEV QQQLAPPPPG HSAFAPEFQQ TDSGKVLSKL QARLDDLWED ITHSLHDQGH SHLGDP |
ApoA5 is an important regulator of serum triglyceride concentrations. An ApoA5 mouse nock-out model produced an approximately four fold increase in serum triglycerides, whereas a knock-in model human ApoA5 produced 50-70% lower concentrations of mouse serum triglycerides. Furthermore, peroxisome proliferators-activated receptoragonists, which are used clinically to lower serum triglyceride concentrations, cause increased ApoA5 mRNA expression. There is very little known about ApoA5 protein in human serum. Lately, it was established that ApoA5 is present in the human serum and it can be detected by polyclonal antibodies against both the HN2 and COOH termini, but at much lower concentration than other apolipoproteins.
APOA5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 366 amino acids (24-366 a.a.) and having a molecular mass of 41.3kDa.APOA5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | APOA5: Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Interacts with GPIHBP1. Up-regulated by PPARA agonists, which are used clinically to lower serum TG (such as fibrates). Liver and plasma. Belongs to the apolipoprotein A1/A4/E family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted; Lipid-binding Chromosomal Location of Human Ortholog: 11q23 Cellular Component: chylomicron; extracellular region; extracellular space Molecular Function:cholesterol binding; cholesterol transporter activity; enzyme activator activity; enzyme binding; heparin binding; lipid binding; low-density lipoprotein receptor binding; phosphatidylcholine binding; phospholipid binding Biological Process: acylglycerol homeostasis; cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; lipid transport; lipoprotein metabolic process; neurite regeneration; phosphatidylcholine metabolic process; phospholipid efflux; positive regulation of fatty acid biosynthetic process; positive regulation of lipid catabolic process; positive regulation of lipoprotein lipase activity; positive regulation of receptor-mediated endocytosis; regulation of cholesterol absorption; reverse cholesterol transport; tissue regeneration; triacylglycerol catabolic process; triacylglycerol metabolic process Disease: Hyperlipoproteinemia, Type V; Hypertriglyceridemia, Familial |
| NCBI Summary: | The protein encoded by this gene is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Oct 2009] |
| UniProt Code: | Q6Q788 |
| NCBI GenInfo Identifier: | 60391728 |
| NCBI Gene ID: | 116519 |
| NCBI Accession: | Q6Q788.1 |
| UniProt Secondary Accession: | Q6Q788,Q3MIK6, Q6UWK9, Q9UBJ3, B0YIV9, |
| UniProt Related Accession: | Q6Q788 |
| Molecular Weight: | 41,213 Da |
| NCBI Full Name: | Apolipoprotein A-V |
| NCBI Synonym Full Names: | apolipoprotein A5 |
| NCBI Official Symbol: | APOA5�� |
| NCBI Official Synonym Symbols: | RAP3; APOAV�� |
| NCBI Protein Information: | apolipoprotein A-V |
| UniProt Protein Name: | Apolipoprotein A-V |
| UniProt Synonym Protein Names: | Apolipoprotein A5; Regeneration-associated protein 3 |
| Protein Family: | Apolipoprotein |
| UniProt Gene Name: | APOA5�� |
| UniProt Entry Name: | APOA5_HUMAN |
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