The Human APOB (Apolipoprotein B) CLIA Kit is a cutting-edge diagnostic tool designed for the accurate measurement of apolipoprotein B levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit delivers reliable and reproducible results, making it an essential asset for a variety of research applications.Apolipoprotein B is a crucial protein involved in lipid transport and metabolism, playing a key role in conditions such as cardiovascular diseases and dyslipidemia.
Detection of apolipoprotein B levels can provide valuable insights into an individual's risk for heart disease and guide personalized treatment strategies.This innovative CLIA kit offers researchers and healthcare professionals a powerful tool for studying the role of apolipoprotein B in health and disease, ultimately paving the way for improved diagnostics and therapies in the field of lipid disorders.
Product Name:
Human ApoB (Apolipoprotein B) CLIA Kit
SKU:
HUES00331
Target:
Human ApoB (Apolipoprotein B)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ApoB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ApoB. You can calculate the concentration of Human ApoB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
95-110
90-107
Average (%)
102
101
98
1:4
Range (%)
92-107
101-112
86-100
Average (%)
98
106
91
1:8
Range (%)
92-105
100-114
88-101
Average (%)
97
107
93
1:16
Range (%)
93-104
85-97
94-110
Average (%)
99
91
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-104
95
EDTA plasma (n=5)
96-108
103
Cell culture media (n=5)
90-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.88
133.55
375.2
51.94
142.52
342.8
Standard deviation
5.83
12.27
29.34
5.98
14.92
29.76
C V (%)
10.62
9.19
7.82
11.51
10.47
8.68
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ApoB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoB in samples. No significant cross-reactivity or interference between Human ApoB and analogues was observed.
