The Human ARG1 (Arginase I) ELISA Kit is a highly reliable and sensitive tool for the quantification of arginase I levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it well-suited for a variety of research applications.Arginase I is an enzyme that plays a key role in the urea cycle and is involved in the metabolism of arginine. Dysregulation of arginase I has been linked to various diseases including cancer, cardiovascular disorders, and immune system dysfunction. Thus, measuring arginase I levels can provide valuable insights into the pathophysiology of these conditions and aid in the development of potential therapies.Overall, the Human ARG1 ELISA Kit offers researchers a powerful tool for studying the role of arginase I in health and disease, with the precision and reliability needed for meaningful scientific discoveries.
Product Name:
Human ARG1 (Arginase I) ELISA Kit
SKU:
HUES01696
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
