The Human BAX (BCL-2 associated X protein) ELISA Kit from Assay Genie is designed for the accurate quantification of BAX levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and consistent results, making it an invaluable tool for a variety of research applications. BAX is a key pro-apoptotic protein that plays a crucial role in regulating programmed cell death.
Dysregulation of BAX has been implicated in various diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions. As such, BAX serves as a critical biomarker for understanding these diseases and developing potential therapeutic interventions. Invest in the Human BAX ELISA Kit from Assay Genie to advance your research and gain valuable insights into the role of BAX in health and disease.
Product Name:
Human Bax (Bcl-2 Associated X Protein) ELISA Kit
SKU:
HUES01746
Target:
Human Bax (Bcl-2 Associated X Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BAX. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BAX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BAX, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BAX. You can calculate the concentration of Human BAX in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
96-113
88-102
Average (%)
101
104
95
1:4
Range (%)
88-101
80-91
84-97
Average (%)
93
86
90
1:8
Range (%)
87-101
86-99
83-95
Average (%)
93
91
89
1:16
Range (%)
92-108
86-101
87-100
Average (%)
99
92
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
99
EDTA plasma (n=5)
88-102
93
Cell culture media (n=5)
89-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.37
4.27
18.72
2.53
4.3
17.45
Standard deviation
0.15
0.24
0.88
0.13
0.19
0.58
C V (%)
6.33
5.62
4.7
5.14
4.42
3.32
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human BAX concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BAX in samples. No significant cross-reactivity or interference between Human BAX and analogues was observed.
